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If you buy a brand new Tuner then it works with 1 car out of the box, unsure about 2nd hand ones.
I'm actually interested in the answer to this question myself as I'm in exactly the same boat.
Might be worth dropping Henk an email at info@fastchip.nl
If either of us gets the answer first, post it here.
I believe it wont as I think the unlock code is to unlock the RS-Tuner from the previous car to your car, thus a new code would be needed. Let us know if this is wrong though